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gene exp calm3 hs00968732 g1  (Thermo Fisher)
94
Thermo Fisher gene exp calm3 hs00968732 g1
(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and <t>CALM3</t> in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Gene Exp Calm3 Hs00968732 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calm3/bio_rxiv__2025__07__25__666838-92-24-8?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
gene exp calm3 hs00968732 g1 - by Bioz Stars, 2026-08
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calm3 sh 1 5 shrnas  (OriGene)
92
OriGene calm3 sh 1 5 shrnas
(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and <t>CALM3</t> in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Calm3 Sh 1 5 Shrnas, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calm3/10__1161_slash_circep__123__012036-57-7-13?v=OriGene
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calm3 sh 1 5 shrnas - by Bioz Stars, 2026-08
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polyclonal antibody against calm3  (ABclonal Biotechnology)
90
ABclonal Biotechnology polyclonal antibody against calm3
(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and <t>CALM3</t> in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Polyclonal Antibody Against Calm3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calm3/pm37839542-84-12-14?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
polyclonal antibody against calm3 - by Bioz Stars, 2026-08
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gene exp calm3 hs00270914 m1  (Thermo Fisher)
86
Thermo Fisher gene exp calm3 hs00270914 m1
(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and <t>CALM3</t> in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Gene Exp Calm3 Hs00270914 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calm3/pm37098502-94-24-27?v=Thermo+Fisher
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gene exp calm3 hs00270914 m1 - by Bioz Stars, 2026-08
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plasmids dna sequences encoding the mice nol12, coa3 and calm3 genes  (Twist Bioscience)
90
Twist Bioscience plasmids dna sequences encoding the mice nol12, coa3 and calm3 genes
(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and <t>CALM3</t> in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Plasmids Dna Sequences Encoding The Mice Nol12, Coa3 And Calm3 Genes, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids dna sequences encoding the mice nol12, coa3 and calm3 genes - by Bioz Stars, 2026-08
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pgal4ad 768 881  (Addgene inc)
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Addgene inc pgal4ad 768 881

Pgal4ad 768 881, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirnas against calm1, calm2, and calm3 mrna  (Thermo Fisher)
90
Thermo Fisher sirnas against calm1, calm2, and calm3 mrna

Sirnas Against Calm1, Calm2, And Calm3 Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calm3 grna2  (Addgene inc)
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calm3 grna  (Addgene inc)
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Addgene inc calm3 grna

Calm3 Grna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and CALM3 in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.

Journal: bioRxiv

Article Title: Potassium channels mediate the inhibitory effect of exosomes on anti-tumor immunity in head and neck cancer

doi: 10.1101/2025.07.25.666838

Figure Lengend Snippet: (A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and CALM3 in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.

Article Snippet: RT-qPCR was conducted utilizing TaqMan Gene Expression Assays (ThermoFisher) to quantify KCNA3 (Hs00704943_s1), KCNN4 (Hs01069779_m1), ORAI1 (Hs03046013_m1), STIM1 (Hs00963373_m1), CALM1 (Hs00300085_s1), CALM2 (Hs00830212_s1), CALM3 (Hs00968732_g1), with 1 8S rRNA (Hs99999901_s1) serving as the reference control.

Techniques: Control, MANN-WHITNEY, Quantitative RT-PCR, Flow Cytometry, Expressing, Fluorescence, Isolation, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila

doi: 10.1016/j.celrep.2021.109700

Figure Lengend Snippet:

Article Snippet: pGAL4AD 768–881 , This work, available from AddGene , Cat# 165820.

Techniques: Virus, Recombinant, Purification, Gel Extraction, Plasmid Preparation, Software